O uso do própolis verde e seus efeitos em modelo animal de aderência peritoneal.

Abstract

Postoperative peritoneal adhesions have great socioeconomic impact and can cause symptoms like chronic pain, female infertility, bowel obstruction and death. The adhesions formation occurs mainly due to peritoneal trauma, inflammatory stimuli and free radicals. As there is no definitive treatment to prevent adhesions and propolis has anti-inflammatory and antioxidant effects, it was assumed for this study, a potential beneficial action of propolis in the prevention of peritoneal adhesions. One of the possible reasons for the difficulty in developing an effective treatment is the lack of an efficient animal model for the induction of peritoneal adhesions, associated with the absence of a clear method for comparing peritoneal adhesions. The objective of this study was to evaluate the effect of the administration of aqueous extract of green propolis, on the formation of peritoneal adhesions, in rats submitted to the animal model of gastrotomy followed by gastrorrhaphy. This required the creation, characterization and validation of a new animal model of gastrotomy followed by gastrorrhaphy as an efficient model to induce the formation of peritoneal adhesions and the creation, characterization and validation of a new quantifiable method of macroscopic classification of peritoneal adhesions. Fifty-three male rats (Rattus norvegicus) were used and an aqueous extract of green propolis, similar to EPP-AF®, was used both per os (gavage) and topically (intraperitoneal), evaluating its action in the process of formation of peritoneal adhesions in rat. The animals treated with intraperitoneal propolis had more extensive adhesions when compared to the animals submitted to gastrotomy followed by gastrorrhaphy without any treatment (surgery group). The animals treated with propolis per os at a dosage of 100 mg/kg/day showed a reduction in peritoneal adhesions compared with the surgery group. The gastrotomy followed by gastrorrhaphy model, proved to be effective in forming adhesions in all animals and all the adhesions formed had the same pattern. The new quantifiable method of macroscopic classification of peritoneal adhesions proved to be effective and reliable to evaluate and compare the alterations found on the peritoneal cavities.